Paper Chromatography Introduction The purpose of this experiment is to observe how chromatography can be used to separate mixtures of chemical substances.
Then About mg of each of the four known compounds will be added to the appropriate test tube, and mg of the unknown will be added to the test tube marked 3-unk.
The test tube with unknown will receive 1. Any insoluble material will be allowed to settle. Capillary tubes will be used to spot the samples of 1-asp, 2-ace, and 3-unk on the coated side of one plate.
The spots are to be evenly spaced, 0. The plate will be viewed under UV light to make sure that enough of each sample has been applied. Capillary tubes will also be used to spot 3-unk, 4-caf, and 5-ibu on the other plate in the same manner.
Again, UV light will be used to make sure enough of each sample has been applied. Then, each TLC plate will be placed spotted end down into a developing jar containing a pool of ethyl acetate that is about 0.
The jar will be capped and when the ethyl acetate rises within cm of the top of the TLC plate, the TLC plate will be removed from the jar and allowed to dry. Once dry, the TLC plate will be analyzed under the UV light and the appearance of the spots will be drawn in a laboratory notebook. Data First, five clean test tubes were labeled 1-asp, 2-ace, 3-unk, 4-caf, and 5-ibu.
A spatula was used to help mix any solids that were having trouble dissolving.
Micro capillary tubes were used to spot 1-asp, 2-ace, and 3-unk on the coated side of one plate. The spots were evenly spaced and about 1 cm from the bottom of the plate and 0. On the second plate, 3-unk, 4-caf, and 5-ibu were plated in the same manner.
The plates were then placed spotted side down in a developing jar that contained a 0. The lid of the jar was screwed on and the plates were allowed to sit in the jar until the ethyl acetate rose to about 2 cm from the top of the plate.
The plates were then removed from the jar and then analyzed under an ultraviolet light.
The appearance and measurements of the spots were recorded in a lab notebook.Thin Layer Chromatography (TLC) is a sensitive, fast, simple and inexpensive analytical technique. It is a micro technique; as little as 10 -9 g of material can be . Lab Reports Lab Report on TLC analisys of Analgestic Drugs A line was drawn 1 CM from the bottom of each plate, and five small, evenly paced marks were made along those lines (see Figure 1).
Thin-layer chromatography (TLC) is an extremely valuable analytical technique in the second, you will analyze the compounds you separated by extraction in the last lab. Principles of TLC. Experiment 6 Fall 3 Experiment A. Plant pigments. Pre-Lab: 1. Prepare your notebook.
Include: name, partner’s name, title of experiment, date, purpose, structures of all reference compounds, procedures and tables for your data. TLC Analysis of Analgesic Drugs-Lab Report 1 - Download as Word Doc .doc /.docx), PDF File .pdf), Text File .txt) or read online. Lab report of TLC analysis.
Thin layer chromatography. this is used to determine the composition of various over the counter drugs. Lab 1 - Thin Layer Chromatography Objective In this laboratory you will separate spinach pigments using thin layer chromatography (TLC).